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How the composition and configuration of the landscape can influence biodiversity is a critical issue in strategic planning and sustainable management of forests. The boreal forest in Quebec has been affected by habitat fragmentation by forestry and natural disturbances. One of its principal biodiversity components is bryophytes, and because they are sensitive to environmental changes, they represent a tool to evaluate the impacts of landscape fragmentation in the boreal forest. This work aims to analyze how the landscape composition (the type of forest, age, density) and its configuration (arrangement of forest patches) can affect the diversity (?, ?, and ?) of boreal bryophytes (mosses and liverworts). We analyzed the diversity of bryophytes from 130 forest patches immersed in landscapes with a gradient of natural and anthropogenic disturbances and forest patches with different structures and compositions. The results will propose thresholds at which the landscape can be modified without compromising diversity.